
The radiopacity of BioCement and Biodentine was 5.00 and 3.92 mmAl, respectively (p 90% cell viability with cell proliferation. BioCement’s and Biodentine’s setting time and compressive strength were not different. The main components of BioCement and Biodentine were calcium and silicon. Once confirmed, the physical properties and pH data were analyzed using the independent t-test, and the biological property data were analyzed using one way ANOVA and Tukey’s multiple comparisons test at a 5% significance level. The data were tested for a normal distribution. Alizarin red staining was performed to detect osteogenic differentiation. Cell migration was examined using a wound healing assay.
#Cement compositions iso
The 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-2 H-tetrazolium hydroxide assay was used to evaluate cell cytotoxicity following ISO 10993-5 2009. The biological properties were assessed using extraction medium from BioCement and Biodentine on human dental pulp cells (hDPCs) in vitro. The physical properties were evaluated following the International Organization for Standardization (ISO) 9917-1 20 2012. MethodsīioCement was prepared from cockle shells and rice husk ash and its chemical composition was determined by X-ray diffraction and X-ray fluorescence spectroscopy.

The aim of this study was to evaluate and compare the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shell (BioCement) with those of a commercial tricalcium silicate cement (Biodentine).

To avoid the environmental impact of mining, calcium carbonate can be obtained from biological sources, such as shelled mollusks, one of which is cockle shell. Calcium carbonate, which is manufactured from limestone, is one of the substrates of tricalcium silicate. Tricalcium silicate is the main component of commercial bioceramic cements that are widely used in endodontic treatment.
